Aktivitas Sucrose Phosphate Synthase dan Kandungan Sukrosa pada Tanaman Tebu Trangenik Hasil Perbanyakan Secara Vegetatif
Abstract
Sugar Cane (Saccharum officinarum L) is one of the estate commodity which developed because ability in sucrose synthesis and sucrose accumulation. Sucrose Phosphate Synthase (SPS) is a key enzym of sucrose biosynthesis in cytosol. SPS gene transformation was expected increase sucrose synthesis and accumulation in sugar cane. Genetic stability of transgenic sugar cane was studied on the several generation. The objective of the research investigated the existence of CaMV promoter, SPS activity and sucrose in transgenic sugar cane leaf after three periode of derived vegetative propagation.
This research was divided into four phase, included DNA isolation, SPS activity, sucrose analysis in sugar cane leaf and yield analysis after 11 month of sugar cane. The result showed that the 25,47% of transgenic sugar cane expressed CaMV promoter by PCR analysis. SPS activity and sucrose content in transgenic sugar cane leaf were higher more than control (r = 0,364). transgenic sugar cane maturely was linger more than control.
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References
Anderson, J.W and J. Beardall. 1991. Molekuler Activities of Plant Cell: An Introduction to Plant Biochemistry. Blackwell Scientific Publications. London
Arencibia, A.D., E.R. Carmida, E. Menendez, and P. Molina. 2000. Transgenic Sugarcane (Saccharum spp). Biotechnology in agriculture and forestry 46: 188 – 206
Buchanan, B., W. Gruissem, and R.L. Johans. 2000. Biochemistry and Molekuler Biology of Plant. Plant Physiologist. America.
Chen, S., M. Hajirezaei, M. Peisker, H. Tschiersch, U. Sonnewald, and F. Bornke. 2005. Decreased Sucrose-6-Phosphatase Level in Transgenic Tobacco Inhibits Photosynthesis, alters Carbohydrate Partitioning, and Reduces Growth. Planta 221: 479 – 492
Gallo Meagher, M. Irvine, J.E. 1996. Herbicide recistant transgenic sugarcane plants containing the bargene. Crop Science 36: 1367 - 1374
Grof, C., Glassop, D., Quick, W., Sonnelwald, U. and Campbell, J. 1998. Molecular Manipulation of Sucrose Phosphate Synthase Activity in Sugarcane. CSIRO Division Tropical Crop and Pasture. Brisbane. Australia. 124-126
Herrera., Estrella, L.R. 2000. Genetically Modified Crops and Developing Countries. Plant Physiol. 124: 923 – 925
Huber, S.C and J. L. Huber. 1992. Role of Sucrose Phosphate Synthase in Sucrose Methabolism in leaves. Plants Physiology 89: 518 – 524
Huber, S. C, Nielsen, T.H., J.L Huber, A Phar, D.M. 1989. Variation among Species in Light Activation of Sucrose Phosphate Synthase. Plant and Cell Physiology 30: 277 – 285
Horvath, H., L. G. Jensen, O. T. Wong, E. Kohl, S. E. Ullich, J. Cochran, C.G. Kannangara, and D. Von Wettstein. 2001. Stability of Transgene Expression Field Performance and Recombination breeding of Transformed Barley Lines. Theor. Appl. Genet 102: 1 -11
Jiang, J., S.D. Linsconbe, J. Wang, and J.H. Oard. 2000. High Efficiensi Trans-formation of US Rice Lines from Mature Seed Drived Calli and Segregation of Glufosinate Resistensi Underfield Conditions. Crop Sci 40: 1729 – 1741
Leon, P., J. Sheen. 2003. Sugar and Hormone Connections Trends. Plant Sci 8: 110 -116
Liu, M.C. 1990. Selection of Agronomically Useful Mutants Through Plant Tissue Culture System. Taiwan Sugar 37 : 8 – 13
Matsuoka, Makoto, Osamu Ideta, Massahito Tani, P., Atuhi Hayakawa and Harufuni Miwa. 2001. Agrobacterium tumefaciens Mediated Transformation of Sugarcane Using Cell Suspension Culture with a Novel Method. Proc. Int. Soc. Sugarcane Technol 24 : 660 – 662
Mirzawan. 2007. Industri Gula Antisipasi Dampak Kekeringan. Raton 15: 22 – 23
Miswar. 2007. Peningkatan Biosintesis Sukrosa Tanaman Tebu (Saccharum officinarum L) melalui Overekspresi Gen Sucrose Phosphate Synthase (SPS). Disertasi. Universitas Gajah Mada. Jogjakarta
Muladno. 2002. Seputar Teknologi Rekayasa Genetika . Pustaka Wirausaha Muda. Bogor
Muller, A.E, Kamisugi Y, Gruneberg R, Niedenhof I, Harold R.J, Meyer P. 1999. Poliandromic sequence and ACT-rich DNA elements promote illegitimate recombination in Nicotiana tabacum. Journal of Moleculer Biology 291: 29 - 46
Nasir, M. 2001. Bioteknologi Potensi dan Keberhasilannya dalam Bidang Pertanian. Raja Grafindo Persada. Jakarta
Rasco-Gaunt S, Riley A, Cammell M, Barcelo P, Lazzeri P.A. 2001. Procedures allowing the transformation of range of European elite Wheat (Triticum aestivum L) varietas via Particle bombardment. Journal of Experimental Botany 52: 865 – 874
Salisbury, F.B and C.W. Ross. 1995. Plant Physiology. Wadsworth Publishing Company. Belmont Californic
Shrawat, A.K. 2007. Genetic Transformation of cereals mediated by Agrobac-terium: Potential and Problems. Laboratory of Moleculer Genetics, Departement of Biological Sciences University of Alberta. Canada
Smith, C. J. 1993. Carbohydrate Chemystry in Plant Biochemistry ang Moleculer Biology. John Wiley and Sons. Ltd. UK.
Stitt, M. 1990. Fructose-2,6-Bisphosphate as a Regulatory Molecule in Plant. Annu. Rev. Plant Physiol. Plant Mol. Biol. 41: 174 -181.
Stryer, L. 2000. Biokimia. Alihbahasa Mohamad Sadikin. Edisi 4. Vol 1. EGC. Jakarta
Sturmn, A., G. O. Tang. 1999. The Sucrose-Cleaving Enzym of Plant are Crucial for Development, Growth ang Carbon Partitioning. Trends in Plant Sciense 4: 401 – 407
Sumarno, A. Fachri, Sri Utami, Siti Marjayanti. 2000. Penelitian Bahan Baku, Keragaan Tanaman, dan Produktivitas Tebu. Pusat Penelitian Perkebunan Gula Indonesia. Pasuruan
Sugiharto, B. 2001. Identifikasi dan Karakterisasi Multib Bentuk Sucrosa Phosphate Synthase pada Tanaman Tebu. Jurnal Ilmu Dasar 2 : 72 -78
Sugiharto, B. 2003. Overekspresi gen Sucrose Phosphate Synthase untuk meningkatkan Biosintesis Sukrosa pada tanaman tebu. Lap Riset Unggulan Terpadu VIII Bidang Bioteknologi. Jember
Sugiharto, B., H. Sakakibara, Sumadi, T. Sugiyama. 1997. Differential Expression of two genes for Sucrose Phosphate Synthase in Sugarcane: Molekuler Cloning of the DNA, and Comparative Analysis of gene Expression. Plant Cell Physiol 38: 961 -965
Sugiyarta, E. 2008. Penataan Varietas Tebu. Lembaga Pendidikan Perkebunan. Yogyakarta
Tu, J., G. Zhang, K. Datta, C. Xu, Q. Zhang, G.S. Khish and S.K. Datta. 2000. Field Performance of Transgenic Elite Commercial Hybrid Rice Expressing Bacillus thuringiensis 8-endoktoxin. Nat. Biotechnol 18 : 1101 -1104
Arencibia, A.D., E.R. Carmida, E. Menendez, and P. Molina. 2000. Transgenic Sugarcane (Saccharum spp). Biotechnology in agriculture and forestry 46: 188 – 206
Buchanan, B., W. Gruissem, and R.L. Johans. 2000. Biochemistry and Molekuler Biology of Plant. Plant Physiologist. America.
Chen, S., M. Hajirezaei, M. Peisker, H. Tschiersch, U. Sonnewald, and F. Bornke. 2005. Decreased Sucrose-6-Phosphatase Level in Transgenic Tobacco Inhibits Photosynthesis, alters Carbohydrate Partitioning, and Reduces Growth. Planta 221: 479 – 492
Gallo Meagher, M. Irvine, J.E. 1996. Herbicide recistant transgenic sugarcane plants containing the bargene. Crop Science 36: 1367 - 1374
Grof, C., Glassop, D., Quick, W., Sonnelwald, U. and Campbell, J. 1998. Molecular Manipulation of Sucrose Phosphate Synthase Activity in Sugarcane. CSIRO Division Tropical Crop and Pasture. Brisbane. Australia. 124-126
Herrera., Estrella, L.R. 2000. Genetically Modified Crops and Developing Countries. Plant Physiol. 124: 923 – 925
Huber, S.C and J. L. Huber. 1992. Role of Sucrose Phosphate Synthase in Sucrose Methabolism in leaves. Plants Physiology 89: 518 – 524
Huber, S. C, Nielsen, T.H., J.L Huber, A Phar, D.M. 1989. Variation among Species in Light Activation of Sucrose Phosphate Synthase. Plant and Cell Physiology 30: 277 – 285
Horvath, H., L. G. Jensen, O. T. Wong, E. Kohl, S. E. Ullich, J. Cochran, C.G. Kannangara, and D. Von Wettstein. 2001. Stability of Transgene Expression Field Performance and Recombination breeding of Transformed Barley Lines. Theor. Appl. Genet 102: 1 -11
Jiang, J., S.D. Linsconbe, J. Wang, and J.H. Oard. 2000. High Efficiensi Trans-formation of US Rice Lines from Mature Seed Drived Calli and Segregation of Glufosinate Resistensi Underfield Conditions. Crop Sci 40: 1729 – 1741
Leon, P., J. Sheen. 2003. Sugar and Hormone Connections Trends. Plant Sci 8: 110 -116
Liu, M.C. 1990. Selection of Agronomically Useful Mutants Through Plant Tissue Culture System. Taiwan Sugar 37 : 8 – 13
Matsuoka, Makoto, Osamu Ideta, Massahito Tani, P., Atuhi Hayakawa and Harufuni Miwa. 2001. Agrobacterium tumefaciens Mediated Transformation of Sugarcane Using Cell Suspension Culture with a Novel Method. Proc. Int. Soc. Sugarcane Technol 24 : 660 – 662
Mirzawan. 2007. Industri Gula Antisipasi Dampak Kekeringan. Raton 15: 22 – 23
Miswar. 2007. Peningkatan Biosintesis Sukrosa Tanaman Tebu (Saccharum officinarum L) melalui Overekspresi Gen Sucrose Phosphate Synthase (SPS). Disertasi. Universitas Gajah Mada. Jogjakarta
Muladno. 2002. Seputar Teknologi Rekayasa Genetika . Pustaka Wirausaha Muda. Bogor
Muller, A.E, Kamisugi Y, Gruneberg R, Niedenhof I, Harold R.J, Meyer P. 1999. Poliandromic sequence and ACT-rich DNA elements promote illegitimate recombination in Nicotiana tabacum. Journal of Moleculer Biology 291: 29 - 46
Nasir, M. 2001. Bioteknologi Potensi dan Keberhasilannya dalam Bidang Pertanian. Raja Grafindo Persada. Jakarta
Rasco-Gaunt S, Riley A, Cammell M, Barcelo P, Lazzeri P.A. 2001. Procedures allowing the transformation of range of European elite Wheat (Triticum aestivum L) varietas via Particle bombardment. Journal of Experimental Botany 52: 865 – 874
Salisbury, F.B and C.W. Ross. 1995. Plant Physiology. Wadsworth Publishing Company. Belmont Californic
Shrawat, A.K. 2007. Genetic Transformation of cereals mediated by Agrobac-terium: Potential and Problems. Laboratory of Moleculer Genetics, Departement of Biological Sciences University of Alberta. Canada
Smith, C. J. 1993. Carbohydrate Chemystry in Plant Biochemistry ang Moleculer Biology. John Wiley and Sons. Ltd. UK.
Stitt, M. 1990. Fructose-2,6-Bisphosphate as a Regulatory Molecule in Plant. Annu. Rev. Plant Physiol. Plant Mol. Biol. 41: 174 -181.
Stryer, L. 2000. Biokimia. Alihbahasa Mohamad Sadikin. Edisi 4. Vol 1. EGC. Jakarta
Sturmn, A., G. O. Tang. 1999. The Sucrose-Cleaving Enzym of Plant are Crucial for Development, Growth ang Carbon Partitioning. Trends in Plant Sciense 4: 401 – 407
Sumarno, A. Fachri, Sri Utami, Siti Marjayanti. 2000. Penelitian Bahan Baku, Keragaan Tanaman, dan Produktivitas Tebu. Pusat Penelitian Perkebunan Gula Indonesia. Pasuruan
Sugiharto, B. 2001. Identifikasi dan Karakterisasi Multib Bentuk Sucrosa Phosphate Synthase pada Tanaman Tebu. Jurnal Ilmu Dasar 2 : 72 -78
Sugiharto, B. 2003. Overekspresi gen Sucrose Phosphate Synthase untuk meningkatkan Biosintesis Sukrosa pada tanaman tebu. Lap Riset Unggulan Terpadu VIII Bidang Bioteknologi. Jember
Sugiharto, B., H. Sakakibara, Sumadi, T. Sugiyama. 1997. Differential Expression of two genes for Sucrose Phosphate Synthase in Sugarcane: Molekuler Cloning of the DNA, and Comparative Analysis of gene Expression. Plant Cell Physiol 38: 961 -965
Sugiyarta, E. 2008. Penataan Varietas Tebu. Lembaga Pendidikan Perkebunan. Yogyakarta
Tu, J., G. Zhang, K. Datta, C. Xu, Q. Zhang, G.S. Khish and S.K. Datta. 2000. Field Performance of Transgenic Elite Commercial Hybrid Rice Expressing Bacillus thuringiensis 8-endoktoxin. Nat. Biotechnol 18 : 1101 -1104
Published
2013-11-27
How to Cite
UNTARI, Wiwik Sri.
Aktivitas Sucrose Phosphate Synthase dan Kandungan Sukrosa pada Tanaman Tebu Trangenik Hasil Perbanyakan Secara Vegetatif.
AGRIBIOS, [S.l.], v. 11, n. 2, p. 87-100, nov. 2013.
ISSN 2723-7044.
Available at: <https://unars.ac.id/ojs/index.php/agribios/article/view/57>. Date accessed: 26 nov. 2024.
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Articles
This work is licensed under a Creative Commons Attribution 4.0 International License.
